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A process of resection-dependent nonhomologous end joining involving the goddess Artemis

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posted on 2023-06-09, 14:34 authored by Penny Jeggo, Markus Löbrich
DNA double-strand breaks (DSBs) are a hazardous form of damage that can potentially cause cell death or genomic rearrangements. In mammalian G1- and G2-phase cells, DSBs are repaired with two-component kinetics. In both phases, a fast process uses canonical nonhomologous end joining (c-NHEJ) to repair the majority of DSBs. In G2, slow repair occurs by homologous recombination. The slow repair process in G1 also involves c-NHEJ proteins but additionally requires the nuclease Artemis and DNA end resection. Here, we consider the nature of slow DSB repair in G1 and evaluate factors determining whether DSBs are repaired with fast or slow kinetics. We consider limitations in our current knowledge and present a speculative model for Artemis-dependent c-NHEJ and the environment underlying its usage.

Funding

RDF8: Identifying the origin of translocation-prone, ATM dependent DNA double-strand breaks using chromatin immunoprecipitation mass spectrometry; UNIVERSITY OF SUSSEX; RDF8-008

History

Publication status

  • Published

File Version

  • Published version

Journal

Trends in Biochemical Sciences

ISSN

0968-0004

Publisher

Elsevier

Issue

9

Volume

42

Page range

690-701

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2018-08-16

First Open Access (FOA) Date

2018-08-16

First Compliant Deposit (FCD) Date

2018-08-15

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