A process of resection-dependent nonhomologous end joining involving the goddess Artemis

Jeggo, Penny and Löbrich, Markus (2017) A process of resection-dependent nonhomologous end joining involving the goddess Artemis. Trends in Biochemical Sciences, 42 (9). pp. 690-701. ISSN 0968-0004

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Abstract

DNA double-strand breaks (DSBs) are a hazardous form of damage that can potentially cause cell death or genomic rearrangements. In mammalian G1- and G2-phase cells, DSBs are repaired with two-component kinetics. In both phases, a fast process uses canonical nonhomologous end joining (c-NHEJ) to repair the majority of DSBs. In G2, slow repair occurs by homologous recombination. The slow repair process in G1 also involves c-NHEJ proteins but additionally requires the nuclease Artemis and DNA end resection. Here, we consider the nature of slow DSB repair in G1 and evaluate factors determining whether DSBs are repaired with fast or slow kinetics. We consider limitations in our current knowledge and present a speculative model for Artemis-dependent c-NHEJ and the environment underlying its usage.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Subjects: Q Science > QH Natural history > QH0301 Biology > QH0426 Genetics > QH0447 Genes. Alleles. Genome
Depositing User: Penny Jeggo
Date Deposited: 16 Aug 2018 10:43
Last Modified: 02 Jul 2019 14:49
URI: http://sro.sussex.ac.uk/id/eprint/77876

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Project NameSussex Project NumberFunderFunder Ref
RDF8: Identifying the origin of translocation-prone, ATM dependent DNA double-strand breaks using chromatin immunoprecipitation mass spectrometryUnsetUNIVERSITY OF SUSSEXRDF8-008