Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies

Molesti, Eleonora, Wright, Edward, Terregino, Calogero, Rahman, Rafat, Cattoli, Giovanni and Temperton, Nigel J (2014) Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies. Journal of Immunology Research, 2014 (457932). pp. 1-11. ISSN 2314-8861

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Abstract

The increased number of outbreaks of H5 and H7 LPAI and HPAI viruses in poultry has major public and animal health implications. The continuous rapid evolution of these subtypes and the emergence of new variants influence the ability to undertake effective surveillance. Retroviral pseudotypes bearing influenza haemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins represent a flexible platform for sensitive, readily standardized influenza serological assays. We describe a multiplex assay for the study of neutralizing antibodies that are directed against both influenza H5 and H7 HA. This assay permits the measurement of neutralizing antibody responses against two antigenically distinct HAs in the same serum/plasma sample thus increasing the amount and quality of serological data that can be acquired from valuable sera. Sera obtained from chickens vaccinated with a monovalent H5N2 vaccine, chickens vaccinated with a bivalent H7N1/H5N9 vaccine, or turkeys naturally infected with an H7N3 virus were evaluated in this assay and the results correlated strongly with data obtained by HI assay. We show that pseudotypes are highly stable under basic cold-chain storage conditions and following multiple rounds of freeze-thaw. We propose that this robust assay may have practical utility for in-field serosurveillance and vaccine studies in resource-limited regions worldwide.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science > QR Microbiology > QR0355 Virology
Depositing User: Edward Wright
Date Deposited: 14 Aug 2018 08:00
Last Modified: 01 Jul 2019 19:46
URI: http://sro.sussex.ac.uk/id/eprint/77820

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