457932.pdf (1.46 MB)
Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies
journal contribution
posted on 2023-06-09, 14:31 authored by Eleonora Molesti, Edward WrightEdward Wright, Calogero Terregino, Rafat Rahman, Giovanni Cattoli, Nigel J TempertonThe increased number of outbreaks of H5 and H7 LPAI and HPAI viruses in poultry has major public and animal health implications. The continuous rapid evolution of these subtypes and the emergence of new variants influence the ability to undertake effective surveillance. Retroviral pseudotypes bearing influenza haemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins represent a flexible platform for sensitive, readily standardized influenza serological assays. We describe a multiplex assay for the study of neutralizing antibodies that are directed against both influenza H5 and H7 HA. This assay permits the measurement of neutralizing antibody responses against two antigenically distinct HAs in the same serum/plasma sample thus increasing the amount and quality of serological data that can be acquired from valuable sera. Sera obtained from chickens vaccinated with a monovalent H5N2 vaccine, chickens vaccinated with a bivalent H7N1/H5N9 vaccine, or turkeys naturally infected with an H7N3 virus were evaluated in this assay and the results correlated strongly with data obtained by HI assay. We show that pseudotypes are highly stable under basic cold-chain storage conditions and following multiple rounds of freeze-thaw. We propose that this robust assay may have practical utility for in-field serosurveillance and vaccine studies in resource-limited regions worldwide.
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Publication status
- Published
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- Published version
Journal
Journal of Immunology ResearchISSN
2314-8861Publisher
Hindawi Publishing CorporationExternal DOI
Issue
457932Volume
2014Page range
1-11Department affiliated with
- Biochemistry Publications
Full text available
- Yes
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- Yes
Legacy Posted Date
2018-08-14First Open Access (FOA) Date
2018-08-14First Compliant Deposit (FCD) Date
2018-08-13Usage metrics
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