Roshchin, Matvey V, Matlashov, Mikhail E, Jerusalimsky, Victor N, Balaban, Pavel M, Belousov, Vsevolod V, Kemenes, György, Staras, Kevin and Nikitin, Evgeny S (2018) A BK channel–mediated feedback pathway links single-synapse activity with action potential sharpening in repetitive firing. Science Advances, 4 (7). eaat1357 1-8. ISSN 2375-2548

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Abstract

Action potential shape is a major determinant of synaptic transmission, and mechanisms of spike tuning are
therefore of key functional significance. We demonstrate that synaptic activity itself modulates future spikes in
the same neuron via a rapid feedback pathway. Using Ca2+ imaging and targeted uncaging approaches in layer
5 neocortical pyramidal neurons, we show that the single spike–evoked Ca2+ rise occurring in one proximal bouton
or first node of Ranvier drives a significant sharpening of subsequent action potentials recorded at the soma.
This form of intrinsic modulation, mediated by the activation of large-conductance Ca2+/voltage-dependent
K+ channels (BK channels), acts to maintain high-frequency firing and limit runaway spike broadening during repetitive
firing, preventing an otherwise significant escalation of synaptic transmission. Our findings identify a
novel short-term presynaptic plasticity mechanism that uses the activity history of a bouton or adjacent axonal
site to dynamically tune ongoing signaling properties.

Item Type:	Article
Keywords:	synaptic activity, action potential shape, BK channel, feedback pathway, repetitive firing, layer 5 neocortical pyramidal neurons, Ca2+, targeted Ca2+ uncaging, first node of Ranvier, proximal bouton
Schools and Departments:	School of Life Sciences > Neuroscience
Research Centres and Groups:	Sussex Neuroscience
Subjects:	Q Science Q Science > QP Physiology > QP0351 Neurophysiology and neuropsychology Q Science > QP Physiology > QP0351 Neurophysiology and neuropsychology > QP0361 Nervous system Q Science > QP Physiology > QP0351 Neurophysiology and neuropsychology > QP0361 Nervous system > QP0364 Synapses
Related URLs:	Publisher
Depositing User:	George Kemenes
Date Deposited:	13 Aug 2018 10:57
Last Modified:	02 Jul 2019 14:30
URI:	http://sro.sussex.ac.uk/id/eprint/77774

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