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Cry78Aa, a novel Bacillus thuringiensis insecticidal protein with activity against Laodelphax striatellus and Nilaparvata lugens

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posted on 2023-06-09, 14:19 authored by Yinglong Wang, Yonglei Liu, Jie Zhang, Neil CrickmoreNeil Crickmore, Fuping Song, Jiguo Gao, Changlong Shu
Transgenic plants expressing insecticidal proteins originating from Bacillus thuringiensis (Bt) have successfully been used to control lepidopteran and coleopteran pests with chewing mouthparts. However, only a handful of Bt proteins have been identified that have bioactivity against sap sucking pests (Hemiptera), including aphids, whiteflies, plant bugs and planthoppers. A novel Bt insecticidal protein with significant toxicity against a hemipteran insect pest is described here. The gene encoding the 359 amino acid, 40.7 kDa protein was cloned from strain C9F1. After expression and purification of the toxin, its median lethal concentration (LC) values against Laodelphax striatellus and Nilaparvata lugens were determined as 6.89 µg/mL and 15.78 µg/mL respectively. Analysis of the toxin sequence revealed the presence of both Toxin_10 and Ricin_B_Lectin domains.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Journal of invertebrate pathology

ISSN

1096-0805

Publisher

Elsevier

Volume

158

Page range

1-5

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2018-08-01

First Open Access (FOA) Date

2019-07-11

First Compliant Deposit (FCD) Date

2018-08-01

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