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Cry78Aa, a novel Bacillus thuringiensis insecticidal protein with activity against Laodelphax striatellus and Nilaparvata lugens
journal contribution
posted on 2023-06-09, 14:19 authored by Yinglong Wang, Yonglei Liu, Jie Zhang, Neil CrickmoreNeil Crickmore, Fuping Song, Jiguo Gao, Changlong ShuTransgenic plants expressing insecticidal proteins originating from Bacillus thuringiensis (Bt) have successfully been used to control lepidopteran and coleopteran pests with chewing mouthparts. However, only a handful of Bt proteins have been identified that have bioactivity against sap sucking pests (Hemiptera), including aphids, whiteflies, plant bugs and planthoppers. A novel Bt insecticidal protein with significant toxicity against a hemipteran insect pest is described here. The gene encoding the 359 amino acid, 40.7 kDa protein was cloned from strain C9F1. After expression and purification of the toxin, its median lethal concentration (LC) values against Laodelphax striatellus and Nilaparvata lugens were determined as 6.89 µg/mL and 15.78 µg/mL respectively. Analysis of the toxin sequence revealed the presence of both Toxin_10 and Ricin_B_Lectin domains.
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Publication status
- Published
File Version
- Accepted version
Journal
Journal of invertebrate pathologyISSN
1096-0805Publisher
ElsevierExternal DOI
Volume
158Page range
1-5Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2018-08-01First Open Access (FOA) Date
2019-07-11First Compliant Deposit (FCD) Date
2018-08-01Usage metrics
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