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The clinico-pathologic role of microRNAs miR-9 and miR-151-5p in breast cancer metastasis
journal contribution
posted on 2023-06-09, 13:47 authored by Jonathan Krell, Adam E Frampton, Jimmy Jacob, Loredana Pellegrino, Laura Roca-Alonso, Daniel Zeloof, Costi Alifrangis, Jacqueline S Lewis, Long R Jiao, Justin Stebbing, Leandro CastellanoLeandro CastellanoBACKGROUND MicroRNAs (miRNAs) may function as suppressors or promoters of tumor metastasis according to their messenger RNA targets. Previous studies have suggested that miR-9 and miR-151-5p are associated with metastasis in breast cancer and hepatocellular carcinoma, respectively. We aimed to further establish the potential roles of miR-9 and miR-151-5p in tumor invasion and metastasis and investigate their use as biomarkers. METHODS We used quantitative real-time PCR (qRT-PCR) to measure differences in miR-9 and miR-151-5p expression between primary breast tumors and their lymph-node metastases in 194 paired tumor samples from 97 patients. We also correlated expression levels with histologic data to investigate their utility as biomarkers. RESULTS There were no significant differences in miR-9 expression between the primary tumors and lymph nodes; however, miR-151-5p expression was significantly lower in the lymph-node metastases than in their corresponding tumors (p < 0.05). miR-9 levels were elevated in primary breast tumors from patients diagnosed with higher-grade tumors (p < 0.05); however, no differences were observed in miR-151-5p levels between different grades of tumor. Interestingly, miR-9 levels were elevated in invasive lobular carcinomas (ILC) compared with invasive ductal carcinomas (IDC; p < 0.01). CONCLUSIONS In aggregate, these data suggest that miR-151-5p upregulation may suppress metastasis in primary breast tumors. Both miRNAs may serve as useful biomarkers in future clinical trials in breast cancer.
History
Publication status
- Published
Journal
Molecular Diagnosis & TherapyISSN
1179-2000Publisher
Springer VerlagExternal DOI
Issue
3Volume
16Page range
167-172Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2018-06-15Usage metrics
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