Whole genome analysis using microarrays

Waddell, Simon J, Hinds, Jason and Butcher, Philip D (2008) Whole genome analysis using microarrays. In: Parish, Tanya and Brown, Amanda Claire (eds.) Mycobacteria Protocols : Second Edition. Methods in Molecular Biology, 465 . Springler, Totowa, NJ, pp. 1-12. ISBN 978-1-58829-889-8

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The development of microarray technology has allowed the genomes of mycobacteria to be directly compared to identify DNA regions that differ between strains due to deletion, insertion, or sequence divergence. The use of microarrays in comparative genomics has proved to be a valuable tool for comparing both mycobacterial species and strains. We describe here the methodology for comparing two mycobacterial DNA samples by microarray hybridization, from labeling and slide preparation, to DNA microarray analysis options. Further developments in microarray design and methodology promise to ensure that microarrays remain an important resource for comparative genomic studies in the future.

Item Type: Book Section
Schools and Departments: School of Life Sciences > Biochemistry
Brighton and Sussex Medical School > Global Health and Infection
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology > QR0075 Bacteria
Depositing User: Simon Waddell
Date Deposited: 08 May 2012 10:58
Last Modified: 22 Sep 2017 11:26
URI: http://sro.sussex.ac.uk/id/eprint/7649

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