Waddell_Tuberculosis accepted Manuscript 2003.pdf (394.48 kB)
The use of microarray analysis to determine the gene expression profiles of Mycobacterium tuberculosis in response to anti-bacterial compounds
journal contribution
posted on 2023-06-07, 16:22 authored by Simon WaddellSimon Waddell, Richard A Stabler, Ken Laing, Laurent Kremer, Robert C Reynolds, Gurdyal S BesraThe response of Mycobacterium tuberculosis to six anti-microbial agents was determined by microarray analysis in an attempt to define mechanisms of innate resistance in M. tuberculosis. The gene expression profiles of M. tuberculosis after treatment at the minimal inhibitory concentration (MIC) for 4 h with isoniazid, isoxyl, tetrahydrolipstatin, SRI#221, SR1#967 and SR1#9190 were compared to untreated M. tuberculosis. A common response to drug exposure was defined, and this expression profile overlapped with a number of other mycobacterial stress responses recently identified by microarray analysis. Compound-specific responses were also distinguished including a number of putative transcriptional regulators and translocation-related genes. These genes may contribute to the intrinsic resistance of M. tuberculosis to anti-microbial compounds. Further investigation into these mechanisms may elucidate novel pathways contributing to mycobacterial drug resistance and influence anti-mycobacterial drug development strategies.
History
Publication status
- Published
File Version
- Accepted version
Journal
TuberculosisISSN
1472-9792Publisher
ElsevierExternal DOI
Issue
3-4Volume
84Page range
263-274Department affiliated with
- Global Health and Infection Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2012-07-17First Open Access (FOA) Date
2017-01-23First Compliant Deposit (FCD) Date
2017-01-23Usage metrics
Categories
No categories selectedLicence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC