Preliminary evidence of reductive stress in human cytotoxic T-cells following exercise

Wadley, Alex J, Holliday, Adrian, Morgan, Rhys G, Heesom, Kate J, Aldred, Sarah, Peters, Derek M, Bueno, Allain A and Coles, Steven J (2018) Preliminary evidence of reductive stress in human cytotoxic T-cells following exercise. Journal of Applied Physiology, 125 (2). pp. 586-595. ISSN 8750-7587

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Abstract

This study investigated immunophenotypic differences in intracellular thiol redox state of peripheral blood mononuclear cells (PBMCs) isolated from trained (TR, n=9, mean {plus minus} SD: age 28 {plus minus} 5 years; BMI 23.2 {plus minus} 2.6 kg·m; VO 56.9 {plus minus} 6.1 ml·kg·min) and recreationally active (RA, n=11, mean {plus minus} SD: age 27 {plus minus} 6 years; BMI 24.2 {plus minus} 3.7 kg·m; VO 45.1 {plus minus} 6.4 ml·kg·min) participants before and after a maximal aerobic exercise tolerance test. Blood samples were taken before (PRE), during (sample acquired at 70% HRmax), immediately (POST+0) and 15 minutes post-exercise (POST+15). PBMCs were isolated and reduced thiol analysis (fluorescein-5 maleimide (F5M)) by immunophenotype (CD3+, CD4+ and CD8) was performed using flow cytometry. A significant increase in cellular F5M fluorescence was observed in CD3+ T-cells at POST+0, with changes driven to a greater extent by CD8 T-cells (fold change in both groups CD4: +2.3, CD8: +2.8; p<0.05). Further analysis revealed a population of highly reduced CD8 T-cells (CD8T-reduced) that significantly increased from PRE to POST+0 in RA participants only (RA: +272 cell/µL, p<0.05). To further understand these results, CD8T-reduced and CD8T-reduced- cells were analysed for immunophenotype in response to the same exercise protocol (n=6, mean {plus minus} SD: age 24 {plus minus} 5 years; BMI 25.7 {plus minus} 4.1 kg·m; VO 41.33 {plus minus} 7.63 ml·kg·min). CD8T-reduced had significantly less lymphoid homing potential (CCR7) POST+0 compared to PRE. This study is the first to demonstrate that lymphocyte populations become more reductive in response to acute exercise.

Item Type: Article
Keywords: Immunity; Lymphocyte; Physical Activity; Reactive Oxygen; Species Redox
Schools and Departments: School of Life Sciences > Biochemistry
Research Centres and Groups: Haematology Research Group
Depositing User: Rhys Morgan
Date Deposited: 10 May 2018 14:43
Last Modified: 01 Jul 2019 12:01
URI: http://sro.sussex.ac.uk/id/eprint/75697

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