University of Sussex
Browse
Bettini,_Natalia_Lorena.pdf (5.96 MB)

NOS-related natural antisense transcripts: sequence analysis and characterization of expression

Download (5.96 MB)
thesis
posted on 2023-06-07, 16:13 authored by Natalia Bettini
Endogenous nitric oxide (NO) produced by the enzyme NO synthase (NOS) has an important role in a variety of physiological processes. However, NO becomes noxious to cells if produced in excess. Therefore, the production of NO is tightly regulated. A particularly exciting and novel aspect of the regulation of NO signalling is the possibility that the expression of NOS genes is controlled by unconventional mechanisms that depend on the presence of natural antisense transcripts (NATs). In this thesis I investigate the important properties of two distinct NOS-related NATs: Lym-antiNOS2 and Mm-antiNOS1. I show that Lym-antiNOS2 RNA is widely expressed in the CNS of the pond snail Lymnaea stagnalis. Furthermore, I demonstrate that the expression of Lym-antiNOS2 is differentially regulated by training leading to long-term memory formation. Moreover, my results indicate that Lym-antiNOS2 RNA is subjected to peripheral trafficking in neurons. As for Mm-antiNOS1, I find that its expression is restricted to embryonic brain tissue and is almost undetectable in the adult brain of Mus musculus.

History

File Version

  • Published version

Pages

209.0

Department affiliated with

  • Biology and Environmental Science Theses

Qualification level

  • doctoral

Qualification name

  • dphil

Language

  • eng

Institution

University of Sussex

Full text available

  • Yes

Legacy Posted Date

2011-10-18

Usage metrics

    University of Sussex (Theses)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC