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A CREB2-targeting microRNA is required for long-term memory after single-trial learning

Version 2 2023-06-12, 08:50
Version 1 2023-06-09, 12:16
journal contribution
posted on 2023-06-12, 08:50 authored by Sergei Korneev, Dimitris V Vavoulis, Souvik Naskar, Varvara E Dyakonova, Ildiko KemenesIldiko Kemenes, George KemenesGeorge Kemenes
Although single-trial induced long-term memories (LTM) have been of major interest in neuroscience, how LTM can form after a single episode of learning remains largely unknown. We hypothesized that the removal of molecular inhibitory constraints by microRNAs (miRNAs) plays an important role in this process. To test this hypothesis, first we constructed small non-coding RNA (sncRNA) cDNA libraries from the CNS of Lymnaea stagnalis subjected to a single conditioning trial. Then, by next generation sequencing of these libraries, we identified a specific pool of miRNAs regulated by training. Of these miRNAs, we focussed on Lym-miR-137 whose seed region shows perfect complementarity to a target sequence in the 3’ UTR of the mRNA for CREB2, a well-known memory repressor. We found that Lym-miR-137 was transiently up-regulated 1?h after single-trial conditioning, preceding a down-regulation of Lym-CREB2 mRNA. Furthermore, we discovered that Lym-miR-137 is co-expressed with Lym-CREB2 mRNA in an identified neuron with an established role in LTM. Finally, using an in vivo loss-of-function approach we demonstrated that Lym-miR-137 is required for single-trial induced LTM.

Funding

BBSRC; BB/H009906/1

BBSRC; BB/P00766X/1

MRC; MRC/G0400551

History

Publication status

  • Published

File Version

  • Published version

Journal

Scientific Reports

ISSN

2045-2322

Publisher

Nature Publishing Group

Issue

1

Volume

8

Page range

1-12

Article number

a3950

Department affiliated with

  • Neuroscience Publications

Research groups affiliated with

  • Sussex Neuroscience Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2018-02-20

First Open Access (FOA) Date

2018-02-20

First Compliant Deposit (FCD) Date

2018-02-20

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