Wildner, Leticia Muraro, Gould, Katherine A and Waddell, Simon J (2018) Transcriptional profiling mycobacterium tuberculosis from patient sputa. In: Antibiotic Resistance Protocols. Methods in Molecular BIology, 1736 . Humana Press, pp. 117-128. ISBN 9781493976362

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Abstract

The emergence of drug resistance threatens to destroy tuberculosis control programmes worldwide, with resistance to all first-line drugs and most second-line drugs detected. Drug tolerance (or phenotypic drug resistance) is also likely to be clinically relevant over the 6-month long standard treatment for drug-sensitive tuberculosis. Transcriptional profiling the response of Mycobacterium tuberculosis to antimicrobial drugs offers a novel interpretation of drug efficacy and mycobacterial drug-susceptibility that likely varies in dynamic microenvironments, such as the lung. This chapter describes the non-invasive sampling of tuberculous sputa and techniques for mRNA profiling M.tb bacilli during patient therapy to characterise real-world drug actions.

Item Type:	Book Section
Keywords:	Health; Infection; drug discovery; antimicrobial drug resistance; tuberculosis
Schools and Departments:	Brighton and Sussex Medical School > Brighton and Sussex Medical School Brighton and Sussex Medical School > Global Health and Infection
Research Centres and Groups:	Wellcome Trust Brighton and Sussex Centre for Global Health Research
Subjects:	Q Science Q Science > QR Microbiology Q Science > QR Microbiology > QR0075 Bacteria
Depositing User:	Simon Waddell
Date Deposited:	25 Jan 2018 13:10
Last Modified:	29 May 2018 15:11
URI:	http://sro.sussex.ac.uk/id/eprint/73140

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