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Growth and proliferation of human embryonic stem cells on fully synthetic scaffolds based on carbon nanotubes

journal contribution
posted on 2023-06-09, 11:40 authored by Eric Brunner, Izabela Jurewicz, Elena Heister, Azin Fahimi, Bo Chiara, Sear Richard, Peter Donovan, Alan DaltonAlan Dalton
Here we show an industrially scalable and inexpensive method of fabricating entirely synthetic, nonxenogeneic carbon nanotube-based scaffolds by vacuum filtration for the culture of human embryonic stem cells. We show that controlled exposure of carbon nanotubes to sonication and the amount of energy delivered to the dispersion directly impacts the surface properties, allowing for control over the nanotopography of the resulting carbon nanotube films, which in turn has demonstrable effects upon in vitro human embryonic stem cells cultures. By altering the nanotube processing conditions before film fabrication, it is possible to influence cell adherence, proliferation and colony morphology. Such a tunable surface with capabilities of influencing stem cell behaviors, combined with the ability to slow or speed population doubling times, will provide crucial solutions for achieving applications envisioned by stem cell biologists to assist future industrial and clinical implementation of human embryonic stem cells.

History

Publication status

  • Published

File Version

  • Published version

Journal

ACS Applied Materials and Interfaces

ISSN

1944-8244

Publisher

American Chemical Society

Issue

4

Volume

6

Page range

2598-2603

Department affiliated with

  • Physics and Astronomy Publications

Research groups affiliated with

  • Materials Physics Group Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2018-01-19

First Compliant Deposit (FCD) Date

2018-01-19

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