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Growth and proliferation of human embryonic stem cells on fully synthetic scaffolds based on carbon nanotubes
journal contribution
posted on 2023-06-09, 11:40 authored by Eric Brunner, Izabela Jurewicz, Elena Heister, Azin Fahimi, Bo Chiara, Sear Richard, Peter Donovan, Alan DaltonAlan DaltonHere we show an industrially scalable and inexpensive method of fabricating entirely synthetic, nonxenogeneic carbon nanotube-based scaffolds by vacuum filtration for the culture of human embryonic stem cells. We show that controlled exposure of carbon nanotubes to sonication and the amount of energy delivered to the dispersion directly impacts the surface properties, allowing for control over the nanotopography of the resulting carbon nanotube films, which in turn has demonstrable effects upon in vitro human embryonic stem cells cultures. By altering the nanotube processing conditions before film fabrication, it is possible to influence cell adherence, proliferation and colony morphology. Such a tunable surface with capabilities of influencing stem cell behaviors, combined with the ability to slow or speed population doubling times, will provide crucial solutions for achieving applications envisioned by stem cell biologists to assist future industrial and clinical implementation of human embryonic stem cells.
History
Publication status
- Published
File Version
- Published version
Journal
ACS Applied Materials and InterfacesISSN
1944-8244Publisher
American Chemical SocietyExternal DOI
Issue
4Volume
6Page range
2598-2603Department affiliated with
- Physics and Astronomy Publications
Research groups affiliated with
- Materials Physics Group Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2018-01-19First Compliant Deposit (FCD) Date
2018-01-19Usage metrics
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