__smbhome.uscs.susx.ac.uk_sf356_Desktop_DNA extraction and PCR 02.pdf (225.69 kB)
[Protocol] Colony polymerase chain reaction with Schizosaccharomyces pombe
journal contribution
posted on 2023-06-09, 09:20 authored by Jo Murray, Adam WatsonAdam Watson, Antony CarrAntony CarrWhen screening a large number of individual Schizosaccharomyces pombe strains by polymerase chain reaction (PCR), a rapid “colony PCR” approach may be used. Numerous colony PCR protocols are available, and fundamental to them all is that the colony must be fresh (grown overnight) and that as few cells as possible are used. In this protocol, we present three reliable methods for preparing S. pombe cells for colony PCR.
Funding
Replication stalling in a palindrome; G0181; ASSOCIATION FOR INTERNATIONAL CANCER RESEARCH; 10-0273
Smc5/6 and replication fork stability; G0179; MRC-MEDICAL RESEARCH COUNCIL; G0901011
History
Publication status
- Published
File Version
- Accepted version
Journal
Cold Spring Harbor ProtocolsISSN
1559-6095Publisher
Cold Spring Harbor Laboratory PressExternal DOI
Issue
5Volume
2016Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Research groups affiliated with
- Genome Damage and Stability Centre Publications
Full text available
- Yes
Peer reviewed?
- No
Legacy Posted Date
2018-02-13First Open Access (FOA) Date
2018-04-16First Compliant Deposit (FCD) Date
2018-04-16Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC