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[Protocol] Colony polymerase chain reaction with Schizosaccharomyces pombe

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posted on 2023-06-09, 09:20 authored by Jo Murray, Adam WatsonAdam Watson, Antony CarrAntony Carr
When screening a large number of individual Schizosaccharomyces pombe strains by polymerase chain reaction (PCR), a rapid “colony PCR” approach may be used. Numerous colony PCR protocols are available, and fundamental to them all is that the colony must be fresh (grown overnight) and that as few cells as possible are used. In this protocol, we present three reliable methods for preparing S. pombe cells for colony PCR.

Funding

Replication stalling in a palindrome; G0181; ASSOCIATION FOR INTERNATIONAL CANCER RESEARCH; 10-0273

Smc5/6 and replication fork stability; G0179; MRC-MEDICAL RESEARCH COUNCIL; G0901011

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Cold Spring Harbor Protocols

ISSN

1559-6095

Publisher

Cold Spring Harbor Laboratory Press

Issue

5

Volume

2016

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • Yes

Peer reviewed?

  • No

Legacy Posted Date

2018-02-13

First Open Access (FOA) Date

2018-04-16

First Compliant Deposit (FCD) Date

2018-04-16

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