Goodyear, Richard J, Lu, Xiaowei, Deans, Michael R and Richardson, Guy P (2017) A tectorin-based matrix and planar-cell-polarity genes are required for normal collagen-fibril orientation in the developing tectorial membrane. Development, 144. pp. 3978-3989. ISSN 0950-1991
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Abstract
The tectorial membrane is an extracellular structure of the cochlea. It develops on the surface of an epithelium and contains collagen fibrils embedded in a tectorin-based matrix. The collagen fibrils are oriented radially with an apically-directed slant - a feature considered critical for hearing. To determine how this pattern is generated, collagen-fibril formation was examined in mice lacking a tectorin-based matrix, epithelial cilia, or the planar-cell-polarity genes Vangl2 and Ptk7. In wild-type mice, collagen-fibril bundles appear within a tectorin-based matrix at E15.5 and, as fibril-number rapidly increases, become co-aligned and correctly oriented. Epithelial-width measurements and data from Kif3acKO mice suggest, respectively, radial stretch and cilia play little, if any, role in determining normal collagen-fibril orientation, but evidence from tectorin-knockout mice indicates confinement is important. PRICKLE2 distribution reveals the planar-cell-polarity axis in the underlying epithelium is organised along the length of the cochlea and, in mice in which this polarity is disrupted, the apically-directed collagen offset is no longer observed. These results highlight the importance of the tectorin-based matrix and epithelial signals for precise collagen organisation in the tectorial membrane
Item Type: | Article |
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Keywords: | Cochlea, Collagen, Hearing, Inner ear, Planar cell polarity, Tectorial membrane |
Schools and Departments: | School of Life Sciences > Neuroscience |
Research Centres and Groups: | Sussex Neuroscience |
Depositing User: | Guy Richardson |
Date Deposited: | 06 Dec 2017 09:42 |
Last Modified: | 02 Jul 2019 14:31 |
URI: | http://sro.sussex.ac.uk/id/eprint/71811 |
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📧 Request an updateProject Name | Sussex Project Number | Funder | Funder Ref |
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The Tectorial Membrane and the Sensory Hair Bundles of the Inner Ear: Mechanisms of Development and Effects of Deafness-Causing Mutations | G0162 | WELLCOME TRUST | 087737 |