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Mitochondrial DNA replication: a PrimPol perspective

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posted on 2023-06-09, 09:10 authored by Laura BaileyLaura Bailey, Aidan DohertyAidan Doherty
PrimPol, (Primase-Polymerase), the most recently identified eukaryotic polymerase, has roles in both nuclear and mitochondrial DNA maintenance. PrimPol is able to act as a DNA polymerase, with the ability to extend primers and also bypass a variety of oxidative and photo-lesions. In addition, PrimPol also functions as a primase, catalysing the preferential formation of DNA primers in a zinc finger-dependent manner. Although PrimPol’s catalytic activities have been uncovered in vitro, we still know little about how and why it is targeted to the mitochondrion and what its key roles are in the maintenance of this multi-copy DNA molecule. Unlike nuclear DNA, the mammalian mitochondrial genome is circular and the organelle has a number of unique proteins essential for its maintenance, presenting a differing environment within which PrimPol must function. Here, we discuss what is currently known about the mechanisms of DNA replication in the mitochondrion, the proteins that carry out these processes and how PrimPol is likely to be involved in assisting this vital cellular process.

Funding

The role of a novel family of eukaryotic DNA polymerases in mitochondrial DNA replication; G0207; BBSRC; BB/H019723/1

Understanding the role of PrimPol in damage tolerance during genome replication in eukaryotic cells; G1621; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/M008800/1

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Biochemical Society Transactions

ISSN

0300-5127

Publisher

Portland Press

Issue

2

Volume

45

Page range

513-529

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-12-04

First Open Access (FOA) Date

2018-04-01

First Compliant Deposit (FCD) Date

2017-12-04

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