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Fitness effects of spontaneous mutations in picoeukaryotic marine green algae

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posted on 2023-06-09, 08:59 authored by Marc Krasovec, Adam Eyre-WalkerAdam Eyre-Walker, Nigel Grimsley, Christophe Salmeron, David Pecqueur, Gwenael Piganeau, Sophie Sanchez-Ferandin
Estimates of the fitness effects of spontaneous mutations are important for understanding the adaptive potential of species. Here, we present the results of mutation accumulation experiments over 265– 512 sequential generations in four species of marine unicellular green algae, Ostreococcus tauri RCC4221, Ostreococcus mediterraneus RCC2590, Micromonas pusilla RCC299, and Bathycoccus prasinos RCC1105. Cell division rates, taken as a proxy for fitness, systematically decline over the course of the experiment in O. tauri, but not in the three other species where the MA experiments were carried out over a smaller number of generations. However, evidence of mutation accumulation in 24 MA lines arises when they are exposed to stressful conditions, such as changes in osmolarity or exposure to herbicides. The selection coefficients, estimated from the number of cell divisions/day, varies significantly between the different environmental conditions tested in MA lines, providing evidence for advantageous and deleterious effects of spontaneous mutations. This suggests a common environmental dependence of the fitness effects of mutations and allows the minimum mutation/genome/generation rates to be inferred at 0.0037 in these species.

History

Publication status

  • Published

File Version

  • Published version

Journal

G3

ISSN

2160-1836

Publisher

Genetics Society of America

Issue

7

Volume

6

Page range

2063-2071

Department affiliated with

  • Evolution, Behaviour and Environment Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-11-23

First Open Access (FOA) Date

2017-11-23

First Compliant Deposit (FCD) Date

2017-11-23

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