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Substrate-induced phenotypical change of monocytes/macrophages into myofibroblast-like cells: a new insight into the mechanism of in-stent restenosis

journal contribution
posted on 2023-06-07, 15:59 authored by Helen Stewart, A L Guildford, D J Lawrence-Watt, M Santin
Stented coronary angioplasty is the procedure of choice to re-establish patency in obstructed coronary arteries. However, the stent implantation procedure often leads to in-stent restenosis, a process that is characterized by stent strut colonization by macrophages and smooth muscle cells and by neointima formation. The present in vitro study investigates the effect of stent materials on the phenotypical features of monocyte/macrophages. Human peripheral blood monocytes from healthy donors (n = 7) were cultured up to 7 days on substrates mimicking: (i) the stent surface (i.e., electropolished stainless steel), (ii) the de-endothelialized vessel wall (collagen-based extracellular matrix gel), and (iii) thrombus (i.e., fibrin gel). The cells were analyzed by immunocytochemistry for their ability to express alpha-actin, a typical myofibroblast marker, by ELISA to determine PDGF-BB and TGF-beta1 secretion and by PCR to evaluate hyaluronan synthase 1, 2, and 3 genes expression. Data were statistically analyzed by ANOVA (Dunnett's test) and data considered significantly different at p

History

Publication status

  • Published

Journal

Journal of Biomedical Materials Research Part A

ISSN

1549-3296

Publisher

John Wiley & Sons

Issue

2

Volume

90

Page range

465-71

Department affiliated with

  • Clinical and Experimental Medicine Publications

Notes

PubMed ID: 18546184 CODEN: JBMRC

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2011-08-18