DDDT-72530-synthesis-and-characterization-of-a-novel-chemically-designe_122214.pdf (3.68 MB)
Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen
journal contribution
posted on 2023-06-09, 07:53 authored by Mehdi Hajmohammadi, Seyed Davar Siadat, Masoud Ghorbani, Mehdi Shafiee Ardestani, Shahram Teimourian, Vahid Asgari, Reza Ahangari Cohan, Mostafa Hajmohammadi, Akram Hajmohammadi, Ramezan Behzadi, Saeid Rajabnezhad, Nabiollah Namvar AslIn recent years, many experiments have been conducted for the production and evaluation of anticancer glycoconjugated vaccines in developed countries and many achievements have been accomplished with Globo H derivatives. In the current experiment, a new chemically designed triplicate version of (Globo H)3–diethylenetriamine pentaacetic acid (DTPA)–KLH antigen was synthesized and characterized. Immunization with (Globo H)3-DTPA-KLH, a hexasaccharide that is a member of a family of antigenic carbohydrates that are highly expressed in various types of cancers conjugated with DTPA and KLH protein, induced a high level of antibody titer along with an elevated level of IL-4 in mice. Treatment of tumors with the collected sera from immunized mice decreased the tumor size in nude mice as well. None of the immunized mice illustrated any sign of tumor growth after injection of MCF-7 cells compared to the control animals. These findings, based on the newly presented structure of the Globo H antigen, lend exciting and promising evidence for clinical advancement in the development of a therapeutic vaccine in the future.
History
Publication status
- Published
File Version
- Published version
Journal
Drug Design, Development and TherapyISSN
1177-8881Publisher
Dove Medical PressExternal DOI
Volume
9Page range
217-239Department affiliated with
- Biochemistry Publications
Research groups affiliated with
- Pharma Supply Chains and Healthcare Technology Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-09-12First Open Access (FOA) Date
2017-09-12First Compliant Deposit (FCD) Date
2017-09-12Usage metrics
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