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SMC complexes differentially compact mitotic chromosomes according to genomic context

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posted on 2023-06-09, 07:50 authored by Stephanie Andrea Schalbettertter, Anton Goloborodko, Geoffrey Fudenberg, Jon-Matthew Belton, Catrina Miles, Miao Yu, Job Dekker, Leonid Mirny, Jon BaxterJon Baxter
Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is to co-align sister chromatids, while condensin generally compacts mitotic chromosomes. We find strikingly different roles for the two complexes in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosome arms, independently of sister chromatid cohesion. Polymer simulations demonstrate that this role can be fully accounted for through cis-looping of chromatin. Second, condensin is generally dispensable for compaction along chromosome arms. Instead, it plays a targeted role compacting the rDNA proximal regions and promoting resolution of peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that distinct SMC-dependent looping activities are selectively deployed to appropriately compact chromosomes.

Funding

How does Condensin mediate topological change during mitosis?; G0865; Bbsrc-Biotechnology & Biological Sciences Research Council

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Nature Cell Biology

ISSN

1465-7392

Publisher

Nature Publishing Group

Issue

9

Volume

19

Page range

1071-1080

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-09-07

First Open Access (FOA) Date

2018-02-21

First Compliant Deposit (FCD) Date

2017-09-07

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