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In vivo and in vitro knockdown approaches in the avian embryo as a means to study semaphorin signaling

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posted on 2023-06-09, 07:25 authored by Karli Montague, Sarah GuthrieSarah Guthrie, Ivana Poparic
A combination of both in vivo and in vitro techniques is invaluable for studying semaphorin signaling in the avian central nervous system. Here we describe how both types of approaches can be used to compliment each other in order to unravel the role that semaphorins play during embryonic development and elucidate the functional consequences of semaphorin knockdown using RNA interference vectors. We describe and discuss specifically the use of in ovo electroporation and primary oculomotor neuron culture to identify the role of semaphorins in oculomotor neuron migration and assess functional consequences of semaphorin disruption in this system.

History

Publication status

  • Published

ISSN

1064-3745

Publisher

Humana Press

Volume

1493

Page range

403-416

Pages

503.0

Book title

Semaphorin signaling

Place of publication

New York, US

ISBN

9781493964468

Series

Methods in molecular biology

Department affiliated with

  • Neuroscience Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2017-07-27

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