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In vivo and in vitro knockdown approaches in the avian embryo as a means to study semaphorin signaling
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posted on 2023-06-09, 07:25 authored by Karli Montague, Sarah GuthrieSarah Guthrie, Ivana PoparicA combination of both in vivo and in vitro techniques is invaluable for studying semaphorin signaling in the avian central nervous system. Here we describe how both types of approaches can be used to compliment each other in order to unravel the role that semaphorins play during embryonic development and elucidate the functional consequences of semaphorin knockdown using RNA interference vectors. We describe and discuss specifically the use of in ovo electroporation and primary oculomotor neuron culture to identify the role of semaphorins in oculomotor neuron migration and assess functional consequences of semaphorin disruption in this system.
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Publication status
- Published
ISSN
1064-3745Publisher
Humana PressExternal DOI
Volume
1493Page range
403-416Pages
503.0Book title
Semaphorin signalingPlace of publication
New York, USISBN
9781493964468Series
Methods in molecular biologyDepartment affiliated with
- Neuroscience Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2017-07-27Usage metrics
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