Signals from the cerebellum guide the pathfinding of inferior olivary axons

Zhu, Yan, Khan, Khurram and Guthrie, Sarah (2003) Signals from the cerebellum guide the pathfinding of inferior olivary axons. Developmental Biology, 257 (2). pp. 233-248. ISSN 0012-1606

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During development, inferior olivary axons cross the floor plate and project from the caudal to the rostral hindbrain, whence they grow into the cerebellar plate. We have investigated the axon guidance signals involved in the formation of this projection in vitro. When the cerebellar plate was grafted ectopically along the margin of the hindbrain in organotypic cultures, inferior olivary axons could pathfind to the ectopic cerebellum, establishing a topographically normal projection. Following rostrocaudal reversal of a region of tissue in the axon pathway between the inferior olive and the cerebellum, olivary axons still navigated towards the cerebellum. Moreover, olivary axons could cross a bridging tissue explant (spinal cord) to reach a cerebellar explant. In collagen gel cultures of inferior olive explants, olivary axon outgrowth increased significantly in the presence of cerebellar explants and axons deflected towards the cerebellar tissue. These results show that the cerebellum is a source of diffusible axon guidance signals for olivary axons. We also found that, in organotypic cultures, olivary axons which had crossed the floor plate showed an increased tendency to respond to cerebellar cues. Taken together, these results indicate that the cerebellum is the source of cues that are chemoattractant and growth-promoting for inferior olivary axons; prior exposure to the floor plate increases responsiveness to these cues.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Depositing User: Stephanie McGuire
Date Deposited: 12 Jul 2017 11:04
Last Modified: 12 Jul 2017 11:04
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