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Patterning of motor neurons by retinoic acid in the chick embryo hindbrain in vitro
journal contribution
posted on 2023-06-09, 07:08 authored by Sonia Guidato, Camilla Barrett, Sarah GuthrieSarah GuthrieMotor neurons are found throughout the developing chick hindbrain, while somatic motor (SM) neurons develop only in rhombomeres 5 to 8 (r5–8), and in r1. In r2–8 neuroepithelial explants from stage 7–10 embryos cultured in collagen gels, we found that motor neurons were generated throughout r2–8, while SM neuron differentiation was restricted to r5–8, as in vivo. Exposure of such explants to retinoic acid (RA) resulted in SM neuron differentiation throughout r2–8, while inclusion of the mesoderm and endoderm suppressed this effect. In explants with mesoderm/endoderm, RA-dependent SM neuron differentiation in rostral rhombomeres was restored by the application of an inhibitor of the RA-degrading enzyme CYP26. We found that the mesoderm/endoderm (either with or without RA) induced Cyp26 expression in the neuroepithelium in vitro, suggesting that the modulatory effect of CYP26 on RA-dependent patterning might be dependent on local signals.
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Publication status
- Published
Journal
Molecular and Cellular NeuroscienceISSN
1044-7431Publisher
ElsevierExternal DOI
Issue
1Volume
23Page range
81-95Department affiliated with
- Neuroscience Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2017-07-12Usage metrics
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