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Ephrin A/EphA controls the rostral turning polarity of a lateral commissural tract in chick hindbrain

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posted on 2023-06-09, 07:07 authored by Yan Zhu, Sarah GuthrieSarah Guthrie, Fujio Murakami
Most post-crossing commissural axons turn into longitudinal paths to make synaptic connections with their targets. Mechanisms that control their rostrocaudal turning polarity are still poorly understood. We used the hindbrain as a model system to investigate the rostral turning of a laterally located commissural tract, identified as the caudal group of contralateral cerebellar-projecting second-order vestibular neurons (cC-VC). We found that the caudal hindbrain possessed a graded non-permissive/repulsive activity for growing cC-VC axons. This non-permissiveness/repulsion was in part mediated by glycosyl-phosphatidylinositol (GPI)-anchored ephrin A. We further demonstrated that ephrin A2 was distributed in a caudal-high/rostral-low gradient in the caudolateral hindbrain and cC-VC axons expressed EphA receptors. Finally, perturbing ephrin A/EphA signalling both in vitro and in vivo led to rostrocaudal pathfinding errors of post-crossing cC-VC axons. These results suggest that ephrin A/EphA interactions play a key role in regulating the polarity of post-crossing cC-VC axons as they turn into the longitudinal axis.

History

Publication status

  • Published

File Version

  • Published version

Journal

Development

ISSN

0950-1991

Publisher

Company of Biologists

Issue

19

Volume

133

Page range

3837-3846

Department affiliated with

  • Neuroscience Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-07-11

First Open Access (FOA) Date

2017-07-11

First Compliant Deposit (FCD) Date

2017-07-11

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