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PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast

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journal contribution
posted on 2023-06-12, 08:40 authored by Yasukazu Daigaku, Thomas Etheridge, Yuka Nakazawa, Mayumi Nakayama, Adam WatsonAdam Watson, Izumi Miyabe, Tomoo Ogi, Mark Osborne, Antony CarrAntony Carr
PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase d with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling.

Funding

Replication fork stability and fork restart; G0745; MRC-MEDICAL RESEARCH COUNCIL; G1100074-E01/1

Single Molecule Imaging of the DNA Damage Response in Live Cells; G0250; EUROPEAN UNION; 268788

History

Publication status

  • Published

File Version

  • Published version

Journal

PLoS Genetics

ISSN

1553-7390

Publisher

Public Library of Science

Issue

5

Volume

13

Article number

e1006789

Department affiliated with

  • Chemistry Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-05-08

First Open Access (FOA) Date

2017-05-16

First Compliant Deposit (FCD) Date

2017-05-08

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