Establishment and molecular characterisation of seven novel soft-tissue sarcoma cell lines

Salawu, Abdulazeez, Fernando, Malee, Hughes, David, Reed, Malcolm W R, Woll, Penella, Greaves, Claire, Day, Chris, Alhajimohammed, Meshal and Sisley, Karen (2016) Establishment and molecular characterisation of seven novel soft-tissue sarcoma cell lines. British Journal of Cancer, 115 (9). pp. 1058-1068. ISSN 0007-0920

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Background: Soft-tissue sarcomas (STS) are a diverse group of malignancies that remain a diagnostic and therapeutic challenge. Relatively few reliable cell lines currently exist. Rapidly developing technology for genomic profiling with emerging insights into candidate functional (driver) aberrations raises the need for more models for in vitro functional validation of molecular targets.

Methods: Primary cell culture was performed on STS tumours utilising a differential attachment approach. Cell lines were characterised by morphology, immunocytochemistry, proliferation assays, short tandem repeat (STR) and microarray-based genomic copy number profiling.

Results: Of 47 STS cases of various subtypes, half formed adherent monolayers. Seven formed self-immortalised cell lines, including three undifferentiated pleomorphic sarcomas, two dedifferentiated liposarcomas (one of which had received radiotherapy), a leiomyosarcoma and a myxofibrosarcoma. Two morphologically distinct yet genetically identical variants were established in separate cultures for the latter two tumours. All cell lines demonstrated genomic and phenotypic features that not only confirm their malignant characteristics but also confirm retention of DNA copy number aberrations present in their parent tumours that likely include drivers.

Conclusions: These primary cell lines are much-needed additions to the number of reliable cell lines of STS with complex genomics available for initial functional validation of candidate molecular targets.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Brighton and Sussex Medical School
Depositing User: Esme Acton-Stewart
Date Deposited: 07 Mar 2017 11:27
Last Modified: 07 Mar 2017 11:27
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