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Establishment and molecular characterisation of seven novel soft-tissue sarcoma cell lines
journal contribution
posted on 2023-06-09, 05:24 authored by Abdulazeez Salawu, Malee Fernando, David Hughes, Malcolm ReedMalcolm Reed, Penella Woll, Claire Greaves, Chris Day, Meshal Alhajimohammed, Karen SisleyBackground: Soft-tissue sarcomas (STS) are a diverse group of malignancies that remain a diagnostic and therapeutic challenge. Relatively few reliable cell lines currently exist. Rapidly developing technology for genomic profiling with emerging insights into candidate functional (driver) aberrations raises the need for more models for in vitro functional validation of molecular targets. Methods: Primary cell culture was performed on STS tumours utilising a differential attachment approach. Cell lines were characterised by morphology, immunocytochemistry, proliferation assays, short tandem repeat (STR) and microarray-based genomic copy number profiling. Results: Of 47 STS cases of various subtypes, half formed adherent monolayers. Seven formed self-immortalised cell lines, including three undifferentiated pleomorphic sarcomas, two dedifferentiated liposarcomas (one of which had received radiotherapy), a leiomyosarcoma and a myxofibrosarcoma. Two morphologically distinct yet genetically identical variants were established in separate cultures for the latter two tumours. All cell lines demonstrated genomic and phenotypic features that not only confirm their malignant characteristics but also confirm retention of DNA copy number aberrations present in their parent tumours that likely include drivers. Conclusions: These primary cell lines are much-needed additions to the number of reliable cell lines of STS with complex genomics available for initial functional validation of candidate molecular targets.
History
Publication status
- Published
Journal
British Journal of CancerISSN
0007-0920Publisher
Nature Publishing GroupExternal DOI
Issue
9Volume
115Page range
1058-1068Department affiliated with
- BSMS Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2017-03-07Usage metrics
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