University of Sussex
Browse
Bajwa Paper complete.pdf (1.79 MB)

CMV-specific T-cell responses at older ages: broad responses with a large central memory component may be key to long-term survival

Download (1.79 MB)
journal contribution
posted on 2023-06-09, 05:04 authored by Martha Bajwa Joseph, Serena Vita, Rosana Vescovini, Martin Larsen, Paolo Sansoni, Nadia Terrazzini, Stefano Caserta, David Thomas, Kevin DaviesKevin Davies, Helen Smith, Florian KernFlorian Kern
Cytomegalovirus (CMV) infection sometimes causes large expansions of CMV-specific T-cells, particularly in older people. This is believed to undermine immunity to other pathogens and to accelerate immunosenescence. While multiple different CMV proteins are recognized, most publications on age-related T-cell expansions have focused on dominant target proteins, UL83 or UL123, and the T-cell activation marker, IFN-?. We were concerned that this narrow approach might have skewed our understanding of CMV-specific immunity at older ages. We have, therefore, widened the scope of analysis to include in vitro-induced T-cell responses to 19 frequently recognized CMV proteins in young and older healthy volunteers and a group of oldest old, long-term survivors (>85 years of age). Polychromatic flow-cytometry was used to analyze T-cell activation markers (CD107, CD154, IL-2, TNF, IFN-?) and memory phenotype (CD27, CD45RA). The older had on average larger T-cell responses than the young, but, interestingly, response size differences were relatively smaller when all activation markers were considered rather than IFN-? or TNF alone. The oldest old recognized more proteins on average than the other groups and had even bigger T-cell responses than the older with a significantly larger central memory CD4 T-cell component.

Funding

The role of CMV-specific immunity in the association between CMV infection and vascular complicatio; G1198; DUNHILL MEDICAL TRUST; R278/0213

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Journal of Infectious Diseases

ISSN

0022-1899

Publisher

Oxford University Press

Issue

8

Volume

215

Page range

1212-1220

Department affiliated with

  • BSMS Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-02-08

First Open Access (FOA) Date

2018-02-13

First Compliant Deposit (FCD) Date

2017-02-08

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC