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New technologies for examining neuronal ensembles in drug addiction and fear

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posted on 2023-06-09, 04:56 authored by Fabio C Cruz, Eisuke KoyaEisuke Koya, Danielle H Guez-Barber, Jennifer M Bossert, Carl R Lupica, Yavin Shaham, Bruce T Hope
Correlational data suggest that learned associations are encoded within neuronal ensembles. However, it has been difficult to prove that neuronal ensembles mediate learned behaviours because traditional pharmacological and lesion methods, and even newer cell type-specific methods, affect both activated and non-activated neurons. Additionally, previous studies on synaptic and molecular alterations induced by learning did not distinguish between behaviourally activated and non-activated neurons. Here, we describe three new approaches—Daun02 inactivation, FACS sorting of activated neurons and c-fos-GFP transgenic rats — that have been used to selectively target and study activated neuronal ensembles in models of conditioned drug effects and relapse. We also describe two new tools — c-fos-tTA mice and inactivation of CREB-overexpressing neurons — that have been used to study the role of neuronal ensembles in conditioned fear.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Nature Reviews Neuroscience

ISSN

1471-003X

Publisher

Nature Research

Volume

14

Page range

743-754

Department affiliated with

  • Psychology Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-01-27

First Open Access (FOA) Date

2017-01-27

First Compliant Deposit (FCD) Date

2017-01-27

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