A wide field fluorescence lifetime imaging system using a light sheet microscope

Birch, Philip M, Moore, Lamar, Li, Xiaofei, Phillips, Roger, Young, Rupert and Chatwin, Chris (2016) A wide field fluorescence lifetime imaging system using a light sheet microscope. Published in: Popp, Jürgen, Tuchin, Valery V, Matthews, Dennis L and Pavone, Francesco S, (eds.) Proceedings of SPIE 2016 on Biophotonics: Photonic Solutions for Better Health Care; Brussels, Belgium; 4th-7th April 2016. 9887 Society of Photo Optical Instrumentation Engineers ISBN 9781510601321

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Fluorescence lifetime imaging microscopy (FLIM) has allowed scientists to discern information about the chemical properties of biological processes and has become a vital tool in the life sciences and medical research communities. Measuring the spatial lifetime distribution of the fluorophores as well as the intensity distribution enables users to discern vital information about the chemical environment. It however, remains challenging and often involves slow scanning. We present a new microscope system based on light sheet illumination that uses a micro channel plate (MCP) device called a Capacitive Division Imaging Readout (CDIR) which has been developed by Photek Ltd. The device uses an array of capacitors to move the charge site from the MCP to four pre-amplifiers and time-over-threshold discriminators. This camera has the ability to image photons as well as measure the arrival time, enabling high speed FLIM imaging of biological samples.

Item Type: Conference Proceedings
Additional Information: Copyright 2016 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Keywords: light sheet microscope, FLIM, MCP, photo multiplier tube
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Research Centres and Groups: Industrial Informatics and Signal Processing Research Group
Subjects: Q Science > QC Physics > QC0350 Optics. Light
R Medicine > R Medicine (General)
Depositing User: Philip Birch
Date Deposited: 04 May 2016 11:18
Last Modified: 03 Jul 2017 10:51
URI: http://sro.sussex.ac.uk/id/eprint/60754

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