University of Sussex
Browse
GhezziChan.pdf (3.73 MB)

Redox proteomics applied to the thiol secretome

Download (3.73 MB)
journal contribution
posted on 2023-06-09, 01:06 authored by Pietro Ghezzi, Chan Philippe
Significance: Secreted proteins are important both as signaling molecules and potential biomarkers. Recent Advances: Protein can undergo different types of oxidation, both in physiological conditions or under oxidative stress. Several redox proteomics techniques have been successfully applied to the identification of glutathionylated proteins, an oxidative post-translational modification consisting in the formation of a mixed disulfide between a protein cysteine and glutathione. Redox proteomics has also been used to study other forms of protein oxidation. Critical Issues: Because of the highest proportion of free cysteines in the cytosol, redox proteomics of protein thiols has focused, so far, on intracellular proteins. However, plasma proteins, such as transthyretin and albumin, have been described as glutathionylated or cysteinylated. The present review discusses the redox state of protein cysteines in relation to their cellular distribution. We describe the various approaches used to detect secreted glutathionylated proteins, the only thiol modification studied so far in secreted proteins, and the specific problems presented in the study of the secretome. Future Directions: This review focusses on glutathionylated proteins secreted under inflammatory conditions and that may act as soluble mediators (cytokines). Future studies on the redox secretome (including other forms of oxidation) might identify new soluble mediators and biomarkers of oxidative stress.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Antioxidants and Redox Signaling

ISSN

1523-0864

Publisher

Mary Ann Liebert

Issue

7

Volume

26

Page range

299-312

Department affiliated with

  • Clinical and Experimental Medicine Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2016-05-04

First Open Access (FOA) Date

2016-05-06

First Compliant Deposit (FCD) Date

2016-05-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC