Burgess_ja_2015_03973h_revision_markedup.pdf (15.02 MB)
Modular design of self-assembling peptide-based nanotubes
journal contribution
posted on 2023-06-09, 00:48 authored by Natasha C Burgess, Thomas H Sharp, Franziska Thomas, Christopher W Wood, Andrew R Thomson, Nathan R Zaccai, R Leo Brady, Louise SerpellLouise Serpell, Derek N WoolfsonAn ability to design peptide-based nanotubes (PNTs) rationally with defined and mutable internal channels would advance understanding of peptide self-assembly, and present new biomaterials for nanotechnology and medicine. PNTs have been made from Fmoc dipeptides, cyclic peptides, and lock-washer helical bundles. Here we show that blunt-ended a-helical barrels, that is, preassembled bundles of a-helices with central channels, can be used as building blocks for PNTs. This approach is general and systematic, and uses a set of de novo helical bundles as standards. One of these bundles, a hexameric a-helical barrel, assembles into highly ordered PNTs, for which we have determined a structure by combining cryo-transmission electron microscopy, X-ray fiber diffraction, and model building. The structure reveals that the overall symmetry of the peptide module plays a critical role in ripening and ordering of the supramolecular assembly. PNTs based on pentameric, hexameric, and heptameric a-helical barrels sequester hydrophobic dye within their lumens.
History
Publication status
- Published
File Version
- Accepted version
Journal
Journal of the American Chemical SocietyISSN
0002-7863Publisher
American Chemical SocietyExternal DOI
Issue
33Volume
137Page range
10554-10562Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2016-04-07First Open Access (FOA) Date
2018-02-01First Compliant Deposit (FCD) Date
2016-04-07Usage metrics
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