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Monitoring changes of paramagnetically-shifted 31P signals in phospholipid vesicles

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posted on 2023-06-09, 00:15 authored by Rebecca E Joyce, Thomas L Williams, Louise SerpellLouise Serpell, Iain Day
Phospholipid vesicles are commonly used as biomimetics in the investigation of the interaction of various species with cell membranes. In this paper we present a 31P NMR investigation of a simple vesicle system using a paramagnetic shift reagent to probe the inner and outer layers of the lipid bilayer. Time-dependent changes in the 31P NMR signal are observed, which differ whether the paramagnetic species is inside or outside the vesicle, and on the choice of buffer solution used. An interpretation of these results is given in terms of the interaction of the paramagnetic shift reagent with the lipids.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Chemical Physics Letters

ISSN

0009-2614

Publisher

Elsevier

Volume

648

Page range

124-129

Department affiliated with

  • Chemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2016-02-09

First Open Access (FOA) Date

2017-02-08

First Compliant Deposit (FCD) Date

2016-02-09

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