Baldock, Robert A, Day, Matthew, Wilkinson, Oliver J, Cloney, Ross, Jeggo, Penelope A, Oliver, Antony W, Watts, Felicity Z and Pearl, Laurence H (2015) ATM localization and heterochromatin repair depend on Direct Interaction of the 53BP1-BRCT2 domain with γH2AX. Cell Reports, 13 (10). pp. 2081-2089. ISSN 2211-1247
![]() |
PDF
- Published Version
Available under License Creative Commons Attribution-NonCommercial No Derivatives. Download (3MB) |
![]() |
PDF
- Published Version
Restricted to SRO admin only Available under License Creative Commons Attribution. Download (74kB) |
Abstract
53BP1 plays multiple roles in mammalian DNA damage repair, mediating pathway choice and facilitating DNA double-strand break repair in heterochromatin. Although it possesses a C-terminal BRCT2 domain, commonly involved in phospho-peptide binding in other proteins, initial recruitment of 53BP1 to sites of DNA damage depends on interaction with histone post-translational modifications-H4K20me2 and H2AK13/K15ub-downstream of the early γH2AX phosphorylation mark of DNA damage. We now show that, contrary to current models, the 53BP1-BRCT2 domain binds γH2AX directly, providing a third post-translational mark regulating 53BP1 function. We find that the interaction of 53BP1 with γH2AX is required for sustaining the 53BP1-dependent focal concentration of activated ATM that facilitates repair of DNA double-strand breaks in heterochromatin in G1.
Item Type: | Article |
---|---|
Schools and Departments: | School of Life Sciences > Sussex Centre for Genome Damage and Stability |
Depositing User: | Felicity Watts |
Date Deposited: | 04 Jan 2016 15:24 |
Last Modified: | 09 Mar 2021 14:30 |
URI: | http://sro.sussex.ac.uk/id/eprint/58864 |
View download statistics for this item
📧 Request an update