Traylen Pathogens 2015.pdf (454.47 kB)
Identification of Epstein-Barr virus replication proteins in Burkitt’s lymphoma cells
journal contribution
posted on 2023-06-08, 22:59 authored by Chris Traylen, Sharada Ramasubramanyan, Jianmin Zuo, Martin Rowe, Rajei Almohammad, Kate Heesom, Steve M M Sweet, David Matthews, Alison SinclairThe working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications.
Funding
Deciphering the complex mechanisms that reprogram gene expression during Epstein Barr Virus replicat; G0803; MRC-MEDICAL RESEARCH COUNCIL; MR/J001708/1
BBSRC; BB/L018438/1
MRC; G0901755
History
Publication status
- Published
File Version
- Published version
Journal
PathogensISSN
2076-0817Publisher
MDPIExternal DOI
Issue
4Volume
4Page range
739-751Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2015-10-30First Open Access (FOA) Date
2015-10-30First Compliant Deposit (FCD) Date
2015-10-30Usage metrics
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