Fluorescence lifetime estimation method for incomplete decay

Yu, Hongqi and Li, David Day-Uei (2015) Fluorescence lifetime estimation method for incomplete decay. Electronics Letters, 51 (21). pp. 1666-1668. ISSN 0013-5194

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A new incomplete decay signal model is proposed to describe the incomplete decay effects in a time- correlated single-photon counting (TCSPC) based fluorescence lifetime imaging (FLIM) system. Based on this model, we modified a MUltiple SIgnal Classification (MUSIC) algorithm to eliminate the incomplete decay effects. Monte Carlo simulations were carried out to demonstrate the performances of the proposed approach. Simulations show that the proposed method is insensitive to the laser pulse rate and has a larger lifetime dynamic range compared with previously reported approaches. As far as we know, this new method is the first non-fitting method that can resolve incomplete decay effects for multi-exponential decays.

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Subjects: Q Science > QC Physics > QC0350 Optics. Light > QC0450 Spectroscopy
Depositing User: David Day-Uei Li
Date Deposited: 04 Sep 2015 11:21
Last Modified: 03 Jul 2019 02:20
URI: http://sro.sussex.ac.uk/id/eprint/56532

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