Estimation of fluorescence lifetimes via rotational invariance techniques

Yu, Hongqi, Saleeb, Rebecca, Dalgarno, Paul and Li, David Day-Uei (2016) Estimation of fluorescence lifetimes via rotational invariance techniques. IEEE Transactions on Biomedical Engineering, 63 (6). pp. 1292-1300. ISSN 0018-9294

[img] PDF - Published Version
Available under License Creative Commons Attribution.

Download (1MB)


Estimation of signal parameters via rotational invariance techniques is a classical algorithm widely used in array signal processing for direction-of-arrival estimation of emitters. Inspired by this method, a new signal model and a new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multi-exponential fluorescence lifetime imaging (FLIM) experiments. The FLERIT only requires a few time bins of a histogram generated by a time-correlated single photon counting FLIM system, greatly reducing the data throughput from the imager to the signal processing units. As a non-iterative method, the FLERIT does not require initial conditions, prior information nor model selection that are usually required by widely used traditional fitting methods, including nonlinear least square methods or maximum likelihood methods. Moreover, its simplicity means it is suitable for implementations in embedded systems for real-time applications. FLERIT was tested on synthesized and experimental fluorescent cell data showing the potentials to be widely applied in FLIM data analysis.

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Related URLs:
Depositing User: David Day-Uei Li
Date Deposited: 03 Sep 2015 14:40
Last Modified: 02 Jul 2019 20:08

View download statistics for this item

📧 Request an update