The_rate_of_X-ray_induced_DNA_Informa_healthcare_2014.pdf (236.36 kB)
The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaff ected by exposure to 50 Hz magnetic fi elds
journal contribution
posted on 2023-06-08, 22:02 authored by Lisa Woodbine, Jackie Haines, Margaret Coster, Lara Barazzuol, Elizabeth Ainsbury, Zenon Sienkiewicz, Penny JeggoFollowing in utero exposure to low dose radiation (10 – 200 mGy), we recently observed a linear induction of DNA double-strand breaks (DSB) and activation of apoptosis in the embryonic neuronal stem/progenitor cell compartment. No signifi cant induction of DSB or apoptosis was observed following exposure to magnetic fi elds (MF). In the present study, we exploited this in vivo system to examine whether exposure to MF before and after exposure to 100 mGy X-rays impacts upon DSB repair rates. Materials and methods : 53BP1 foci were quantifi ed following combined exposure to radiation and MF in the embryonic neuronal stem/progenitor cell compartment. Embryos were exposed in utero to 50 Hz MF at 300 m T for 3 h before and up to 9 h after exposure to 100 mGy X-rays. Controls included embryos exposed to MF or X-rays alone plus sham exposures. Results : Exposure to MF before and after 100 mGy X-rays did not impact upon the rate of DSB repair in the embryonic neuronal stem cell compartment compared to repair rates following radiation exposure alone. Conclusions : We conclude that in this sensitive system MF do not exert any signifi cant level of DNA damage and do not impede the repair of X-ray induced damage.
History
Publication status
- Published
File Version
- Published version
Journal
International Journal of Radiation BiologyISSN
0955-3002Publisher
Taylor & FrancisExternal DOI
Issue
6Volume
91Page range
495-499Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2015-08-04First Open Access (FOA) Date
2015-08-04First Compliant Deposit (FCD) Date
2015-08-04Usage metrics
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