University of Sussex
Browse

File(s) not publicly available

Broader implications of SILAC-based proteomics for dissecting signaling dynamics in cancer

journal contribution
posted on 2023-06-08, 21:28 authored by Hua Zhang, Yichen Xu, Panos Papanastasopoulos, Justin Stebbing, Georgios GiamasGeorgios Giamas
Large-scale transcriptome and epigenome analyses have been widely utilized to discover gene alterations implicated in cancer development at the genetic level. However, mapping of signaling dynamics at the protein level is likely to be more insightful and needed to complement massive genomic data. Stable isotope labeling with amino acids in cell culture (SILAC)-based proteomic analysis represents one of the most promising comparative quantitative methods that has been extensively employed in proteomic research. This technology allows for global, robust and confident identification and quantification of signal perturbations important for the progress of human diseases, particularly malignancies. The present review summarizes the latest applications of in vitro and in vivo SILAC-based proteomics in identifying global proteome/phosphoproteome and genome-wide protein-protein interactions that contribute to oncogenesis, highlighting the recent advances in dissecting signaling dynamics in cancer.

History

Publication status

  • Published

Journal

Expert Review of Proteomics

ISSN

1478-9450

Publisher

Expert Reviews

Issue

6

Volume

11

Page range

713-731

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2015-07-07

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC