Translesion synthesis in mammalian cells

Lehmann, Alan R. (2006) Translesion synthesis in mammalian cells. Experimental Cell Research, 312 (14). pp. 2673-2676. ISSN 0014-4827

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DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five polymerases used in TLS in human cells have different substrate specificities, enabling them to deal with many different types of damaged bases. PCNA plays a central role in recruiting the TLS polymerases and effecting the polymerase switch from replicative to TLS polymerase. When the fork is blocked PCNA gets ubiquitinated. This increases its affinity for the TLS polymerases, which all have novel ubiquitin-binding motifs, thereby facilitating their engagement at the stalled fork to effect TLS.

Item Type: Article
Keywords: Animals DNA Repair DNA Replication DNA-Directed DNA Polymerase/ physiology Humans Models, Biological Proliferating Cell Nuclear Antigen/ physiology Ubiquitin/metabolism Ultraviolet Rays
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH0301 Biology
Depositing User: Alan Lehmann
Date Deposited: 23 Nov 2006
Last Modified: 02 Jul 2019 21:21
Google Scholar:71 Citations

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