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“Breaking up is hard to do”: the formation and resolution of sister chromatid intertwines

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journal contribution
posted on 2023-06-08, 20:31 authored by Jon BaxterJon Baxter
The absolute necessity to resolve every intertwine between the two strands of the DNA double helix provides a massive challenge to the cellular processes that duplicate and segregate chromosomes. Although the overwhelming majority of intertwines between the parental DNA strands are resolved during DNA replication, there are numerous chromosomal contexts where some intertwining is maintained into mitosis. These mitotic sister chromatid intertwines (SCIs) can be found as; short regions of unreplicated DNA, fully replicated and intertwined sister chromatids—commonly referred to as DNA catenation—and as sister chromatid linkages generated by homologous recombination-associated processes. Several overlapping mechanisms, including intra-chromosomal compaction, topoisomerase action and Holliday junction resolvases, ensure that all SCIs are removed before they can prevent normal chromosome segregation. Here, I discuss why some DNA intertwines persist into mitosis and review our current knowledge of the SCI resolution mechanisms that are employed in both prokaryotes and eukaryotes, including how deregulating SCI formation during DNA replication or disrupting the resolution processes may contribute to aneuploidy in cancer.

Funding

Understanding the mechanisms of termination of DNA replication in eukaryotes Fellow: Baxter; G0222; ROYAL SOCIETY; UO090655

How does Condensin mediate topological change during mitosis?; G0865; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/J018554/1

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Journal of Molecular Biology

ISSN

0022-2836

Publisher

Elsevier

Issue

3

Volume

427

Page range

590-607

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-04-09

First Open Access (FOA) Date

2015-09-04

First Compliant Deposit (FCD) Date

2015-04-09

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