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A global profile of replicative polymerase usage

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posted on 2023-06-08, 20:25 authored by Yasukazu Daigaku, Andrea KeszthelyiAndrea Keszthelyi, Carolin A Müller, Izumi Miyabe, Tony Brooks, Renata Retkute, Mike Hubank, Conrad A Nieduszynski, Antony CarrAntony Carr
Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol) a–primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Pol? replicates the leading strand, whereas Pold performs lagging-strand synthesis. However, it is not known whether this division of labor is maintained across the whole genome or how uniform it is within single replicons. Using Schizosaccharomyces pombe, we have developed a polymerase usage sequencing (Pu-seq) strategy to map polymerase usage genome wide. Pu-seq provides direct replication-origin location and efficiency data and indirect estimates of replication timing. We confirm that the division of labor is broadly maintained across an entire genome. However, our data suggest a subtle variability in the usage of the two polymerases within individual replicons. We propose that this results from occasional leading-strand initiation by Pold followed by exchange for Pol?.

Funding

Replication fork stability and fork restart; G0745; MRC-MEDICAL RESEARCH COUNCIL; G1100074-E01/1

ERC; 268788-SMI-DDR

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Nature Structural and Molecular Biology

ISSN

1545-9993

Publisher

Nature Publishing Group

Issue

3

Volume

22

Page range

192-198

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-03-25

First Open Access (FOA) Date

2015-08-09

First Compliant Deposit (FCD) Date

2015-03-25

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