File(s) not publicly available
Identification of a mosquitocidal toxin from Bacillus thuringiensis using mass spectrometry
journal contribution
posted on 2023-06-08, 20:00 authored by Wenfei Zhang, Jie Zhang, Neil CrickmoreNeil Crickmore, Zhongqi Wu, Yiran Yang, Jiangzhao Qian, Hongping Wu, Ruiping Wang, Xuanjun FangThe Bacillus thuringiensis strain S2160-1 has previously been identified as being highly toxic to mosquito larvae and a viable alternative to strains currently used commercially to control these insects. A PCR approach had identified the presence of four putative insecticidal toxin genes (cry30Ea, cry30 Ga, cry50Ba and cry54Ba) in this strain, but did not identify the genes that encoding three of the main crystal toxin proteins of size 140 and 130 and 30 kDa. In this study we used mass spectrometry to identify the 130 kDa toxin as a rare Cry4 toxin (Cry4Cb3). The gene encoding this toxin was cloned and expressed and the toxin shown to have mosquitocidal activity against Culex quinquefasciatus.
History
Publication status
- Published
Journal
World Journal of Microbiology and BiotechnologyISSN
0959-3993Publisher
Springer VerlagExternal DOI
Issue
12Volume
30Page range
3273-3277Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2015-02-12Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC