Mancini_&_Fuller_(2000).pdf (1.12 MB)
Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus
The recent advances in the resolution obtained by single-particle reconstructions from cryo-electron microscopy (cryo-EM) have led to an increase in studies that combine X-ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single-particle reconstruction, the effect of the correction of the contrast-transfer function on the structure determined and the use of a high-resolution structure of a subunit in the interpretation of the structure of the complex are addressed.
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Publication status
- Published
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- Published version
Journal
Acta Crystallographica Section D: Biological CrystallographyISSN
0907-4449Publisher
International Union of CrystallographyExternal DOI
Issue
10Volume
56Page range
1278-1287Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2015-01-29First Open Access (FOA) Date
2015-01-29First Compliant Deposit (FCD) Date
2015-01-29Usage metrics
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