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Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus

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posted on 2023-06-08, 19:51 authored by Erika ManciniErika Mancini, S D Fuller
The recent advances in the resolution obtained by single-particle reconstructions from cryo-electron microscopy (cryo-EM) have led to an increase in studies that combine X-ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single-particle reconstruction, the effect of the correction of the contrast-transfer function on the structure determined and the use of a high-resolution structure of a subunit in the interpretation of the structure of the complex are addressed.

History

Publication status

  • Published

File Version

  • Published version

Journal

Acta Crystallographica Section D: Biological Crystallography

ISSN

0907-4449

Publisher

International Union of Crystallography

Issue

10

Volume

56

Page range

1278-1287

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-01-29

First Open Access (FOA) Date

2015-01-29

First Compliant Deposit (FCD) Date

2015-01-29

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