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Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus

journal contribution
posted on 2023-06-08, 19:51 authored by Erika ManciniErika Mancini, Mairi Clarke, Brent E Gowen, Twan Rutten, Stephen D Fuller
Semliki Forest virus serves as a paradigm for membrane fusion and assembly. Our icosahedral reconstruction combined 5276 particle images from 48 cryo-electron micrographs and determined the virion structure to 9 Å resolution. The improved resolution of this map reveals an N-terminal arm linking capsid subunits and defines the spike-capsid interaction sites. It illustrates the paired helical nature of the transmembrane segments and the elongated structures connecting them to the spike projecting domains. A 10 Å diameter density in the fusion protein lines the cavity at the center of the spike. These clearly visible features combine with the variation in order between the layers to provide a framework for understanding the structural changes during the life cycle of an enveloped virus.

History

Publication status

  • Published

Journal

Molecular Cell

ISSN

1097-2765

Publisher

Elsevier

Issue

2

Volume

5

Page range

255-266

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2015-01-29

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