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A procedure for setting up high-throughput nanolitre crystallization experiments. II. Crystallization results

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posted on 2023-06-08, 19:50 authored by J Brown, T S Walter, L Carter, N G A Abrescia, A R Aricescu, T D Batuwangala, L E Bird, N Brown, P P Chamberlain, S J Davis, E Dubinina, J Endicott, J A Fennelly, R J C Gilbert, M Harkiolaki, W-C Hon, F Kimberley, C A Love, Erika ManciniErika Mancini, R Manso-Sancho, C E Nichols, R A Robinson, G C Sutton, N Schueller, M C Sleeman, G B Stewart-Jones, M Vuong, J Welburn, Z Zhang, D K Stammers, R J Owens, E Y Jones, K Harlos, D I Stuart
An initial tranche of results from day-to-day use of a robotic system for setting up 100 nl-scale vapour-diffusion sitting-drop protein crystallizations has been surveyed. The database of over 50 unrelated samples represents a snapshot of projects currently at the stage of crystallization trials in Oxford research groups and as such encompasses a broad range of proteins. The results indicate that the nanolitre-scale methodology consistently identifies more crystallization conditions than traditional hand-pipetting-style methods; however, in a number of cases successful scale-up is then problematic. Crystals grown in the initial 100 nl-scale drops have in the majority of cases allowed useful characterization of x-ray diffraction, either in-house or at synchrotron beamlines. For a significant number of projects, full x-ray diffraction data sets have been collected to 3 Å resolution or better (either in-house or at the synchrotron) from crystals grown at the 100 nl scale. To date, five structures have been determined by molecular replacement directly from such data and a further three from scale-up of conditions established at the nanolitre scale.

History

Publication status

  • Published

File Version

  • Published version

Journal

Journal of Applied Crystallography

ISSN

0021-8898

Publisher

International Union of Crystallography

Issue

2

Volume

36

Page range

315-318

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-01-29

First Open Access (FOA) Date

2015-01-29

First Compliant Deposit (FCD) Date

2015-01-29

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