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Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4

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posted on 2023-06-08, 19:50 authored by Rosa Morra, Benjamin M Lee, Heather Shaw, Roman Tuma, Erika ManciniErika Mancini
CHD4, the core subunit of the Nucleosome Remodelling and Deacetylase (NuRD) complex, is a chromatin remodelling ATPase that, in addition to a helicase domain, harbors tandem plant homeo finger and chromo domains. By using a panel of domain constructs we dissect their roles and demonstrate that DNA binding, histone binding and ATPase activities are allosterically regulated. Molecular shape reconstruction from small-angle X-ray scattering reveals extensive domain-domain interactions, which provide a structural explanation for the regulation of CHD4 activities by intramolecular domain communication. Our results demonstrate functional interdependency between domains within a chromatin remodeller. Crown Copyright © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical society. All rights reserved.

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Publication status

  • Published

File Version

  • Published version

Journal

FEBS Letters

ISSN

0014-5793

Publisher

Elsevier

Issue

16

Volume

586

Page range

2513-2521

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-01-30

First Open Access (FOA) Date

2016-03-22

First Compliant Deposit (FCD) Date

2016-11-16

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