Poland, Simon P, Krstajić, Nikola, Coelho, Simao, Tyndall, David, Walker, Richard J, Devauges, Viviane, Morton, Penny E, Nicolas, Nicole S, Richardson, Justin, Li, David Day-Uei, Suhling, Klaus, Wells, Claire M, Parsons, Maddy, Henderson, Robert K and Ameer-Beg, Simon M (2014) A time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo. Optics Letters, 39 (20). pp. 6013-6016. ISSN 0146-9592

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Abstract

Imaging the spatio-temporal interaction of proteins in vivo is essential to understanding the complexities of biological systems. The highest accuracy monitoring of protein-protein interactions is achieved using FRET measured by fluorescence lifetime imaging, with measurements taking minutes to acquire a single frame, limiting their use in dynamic live cell systems. We present a diffraction limited, massively parallel, time-resolved multifocal multiphoton microscope capable of producing fluorescence lifetime images with 55 ps time-resolution, giving improvements in acquisition speed of a
factor of 64. We present demonstrations with FRET imaging in a model cell system and demonstrate in vivo FLIM using a GTPase biosensor in the zebrafish embryo.

Item Type:	Article
Schools and Departments:	School of Engineering and Informatics > Engineering and Design
Subjects:	Q Science > QC Physics > QC0350 Optics. Light > QC0450 Spectroscopy
Depositing User:	David Day-Uei Li
Date Deposited:	06 Oct 2014 09:50
Last Modified:	02 Jul 2019 22:16
URI:	http://sro.sussex.ac.uk/id/eprint/50483

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