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Use of a pooled clone method to isolate a novel Bacillus thuringiensis Cry2A toxin with activity against Ostrinia furnacalis
journal contribution
posted on 2023-06-08, 18:12 authored by Changlong Shu, Jingtao Zhang, Guihua Chen, Gemei Liang, Kanglai He, Neil CrickmoreNeil Crickmore, Dafang Huang, Jie Zhang, Fuping SongA pooled clone method was developed to screen for cry2A genes. This metagenomic method avoids the need to analyse isolated Bacillus thuringiensis strains by performing gene specific PCR on plasmid-enriched DNA prepared from a pooled soil sample. Using this approach the novel holotype gene cry2Ah1 was cloned and characterized. The toxin gene was over-expressed in Escherichia coli Rosetta (DE3) and the expressed toxin accumulated in both the soluble and insoluble fractions. The soluble Cry2Ah1 was found to have a weight loss activity against Ostrinia furnacalis, and a growth inhibitory activity to both Cry1Ac-susceptible and resistant Helicoverpa armigera populations.
History
Publication status
- Published
Journal
Journal of Invertebrate PathologyISSN
0022-2011Publisher
ElsevierExternal DOI
Issue
1Volume
114Page range
31-33Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2014-09-05Usage metrics
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