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Site-specific human histone H3 methylation stability: fast K4me3 turnover

journal contribution
posted on 2023-06-08, 18:03 authored by Yupeng Zheng, Jeremiah D Tipton, Paul M Thomas, Neil L Kelleher, Steve M M Sweet
We employ stable-isotope labeling and quantitative mass spectrometry to track histone methylation stability. We show that H3 trimethyl K9 and K27 are slow to be established on new histones and slow to disappear from old histones, with half-lives of multiple cell divisions. By contrast, the transcription-associated marks K4me3 and K36me3 turn over far more rapidly, with half-lives of 6.8 h and 57 h, respectively. Inhibition of demethylases increases K9 and K36 methylation, with K9 showing the largest and most robust increase. We interpret different turnover rates in light of genome-wide localization data and transcription-dependent nucleosome rearrangements proximal to the transcription start site.

History

Publication status

  • Published

File Version

  • Published version

Journal

Proteomics

ISSN

1615-9853

Publisher

Wiley

Issue

19

Volume

14

Page range

2190-2199

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

Keywords: Cell biology / Chromatin / Demethylation / Heterochromatin / Methylation turnover / SILAC / Transcription

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2014-08-07

First Compliant Deposit (FCD) Date

2014-08-07

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